Bolt, winner of eight Olympic and 11 world gold medals, will bring down the curtain on his glittering career at August's world championships in London.
After kicking off his season on home soil in Kingston, the 30-year-old chose Ostrava and the Diamond League meet in Monaco as his two pre-worlds warm-up events.
It will be Bolt's ninth appearance in the northeastern Czech city, where organiser Alfons Juck has unashamedly gone out of his way to tailor the meet to the Jamaican's plans for the season.
"We gave priority in our preparation and the whole set-up of the meet to Usain," Juck told AFP.
Bolt said a Czech visit was always on the cards. "One meet I was always going to come to was Ostrava," he said.
